Instructions for Mumps Virus Specimen Submission

Reverse transcriptase polymerase chain reaction (RT-PCR) has the greatest diagnostic sensitivity to detect mumps virus when samples are collected within the first nine days of symptoms. Buccal swabs are the preferred specimen and should be collected from a patient as soon as mumps disease is suspected or after contact with a symptomatic individual.

Specimens should be collected and stored at 2-8^oC as heat decreases the stability and infectivity of mumps virus. Specimens must be shipped on multiple cold-packs to maintain consistent temperature during transit. Be aware that storing specimens at ≤ -70^oC is permissible but requires that sample(s) be shipped on dry ice to the testing laboratory. Avoid freeze-thawing of specimen(s) as this also decreases infectivity of the virus.

Specimens MUST be received by the Illinois Department of Public Health laboratory no later than eight days from the date of collection. For any questions pertaining to sample collection, storage, or shipping, contact one of the IDPH laboratories using the contact information below.

(Submission kits can be requested through the Electronic Test Ordering and Reporting (ETOR) system. Contact dph.labs.dmg@illinois.gov for question regarding ETOR enrollment).

1. Do not use expired media or swabs. Store viral transport media (VTM) or universal transport media (UTM) in the refrigerator until use. Use Dacron- or Nylon-tipped swabs for collecting samples. Flocked swabs are preferred. Examples of acceptable swabs include FLOQSwabs™ (Copan) and BD flocked swabs. Do not use cotton-tipped, wooden-shafted, or calcium alginate swabs, as these contain PCR inhibitors. Samples collected using unapproved materials will be rejected from testing.
2. The preferred specimen is a swab collected from the parotid gland duct within nine days from the onset of symptoms.
   
3. Massage the parotid (salivary) glands for 30 seconds and swab specimen from the buccal cavity located between the cheek and teeth of the upper-rear molars.
   
   1. If collecting swabs from the both the right and left parotid gland, combine both swabs into a single tube of viral transport media (VTM).
      
4. Place the swab in 1-3 mL of sterile VTM and break the shaft of the swab at the scored line. Securely close the container.
   
5. Ensure that the patient’s name, date-of-birth, and time/date of collection are recorded on the specimen tube along with the name or initials of the individual collecting the sample.
   
6. Complete all the demographic information on the communicable disease laboratory test requisition form available at https://dph.illinois.gov/topics-services/lab-testing-services/clinical-testing. The authorization number provided by the IDPH Communicable Disease Control Section MUST be included on the requisition form.
   
7. Refrigerate the specimen between 2-8^oC and ship the specimen(s) on cold packs. If the specimen was stored at ≤ -70^oC, the specimen must be shipped on dry ice. Avoid freeze-thawing the specimen.
   
8. The specimen(s) must be received at the laboratory no later than eight days from the date of collection. Do not ship specimens over weekends or holidays as they will not be received until next business day, and cold-packs will not maintain the required 2-8^oC specimen temperature.
   
9. For any questions pertaining to sample collection, storage, or shipping, contact one of the IDPH laboratories using the contact information below.
   

1. Messenger/Courier by ground transport: Place specimen(s) into a biohazard labeled bag and seal securely. Place the test requisition(s) on the outside of the biohazard labeled bag. Place the sealed biohazard bag and test requisition(s) inside the shipping container. Place cold packs, which have been frozen for at least 24 hours, in the leak-proof outer container. The shipping container must be rigid, such as a Styrofoam cooler, and labeled with the UN 3373 Biological Substance Category B marking. Close securely.
2. Commercial carrier by ground/air transport: Place the specimen(s) inside a biohazard labeled bag and seal securely. Place the test requisition(s) on the outside of the biohazard labeled bag. Place the sealed bag and completed test requisitions(s) inside the outer shipping container. Place cold packs, which have been frozen for at least 24 hours, in the leak-proof outer container. Label the outer shipping container with the Illinois Department of Public Health laboratory address listed below. Complete the return address section to include the name of the person shipping the package, business name and address, and a business phone number. The shipping container must include the UN3373 Biological Substance Category B marking.
   
3. Ship specimens by overnight delivery to the attention of the Molecular or Virology lab at the Springfield or Chicago testing laboratories. This can be accomplished by use of local courier, shipping corporations, or U.S. Postal Service.
   
4. The specimen(s) must be received at the laboratory no later than eight days from the date of collection. Do not ship specimens over weekends or holidays as they will not be received until next business day and cold-packs will not maintain the required 2-8^oC specimen temperature.
   

NOTE: Testing may be delayed, or specimens may be considered UNSATISFACTORY if the above instructions are not followed or the requisition form is not filled out completely. If there are any questions about specimen collection, handling, or shipping, contact one of the Illinois Department of Public Health laboratories listed below and speak to molecular laboratory personnel.

Ship specimens by a local courier or overnight by commercial carrier to the designated laboratories indicated below.

Illinois Department of Public Health
Division of Laboratories
ATTN: Molecular Lab
825 N. Rutledge St.
Springfield, IL 62702
Phone 217-782-6562

Illinois Department of Public Health
Division of Laboratories
ATTN: Virology Lab
2121 W. Taylor St.
Chicago, IL 60612
Phone 312-793-4760